IMGM’s digital PCR services – Why life is easier in a binary world
0 or 1, yes or no, true or false – computers love this stuff. An incredibly simple and clear code of binary, unequivocal information units is the basis of their incredible power and range of applications. Digital PCR is ready to follow their footsteps by transforming the vague world of conventional PCR into yes or no information units and thereby unprecedentedly increasing accuracy and application range. Read on and find out how going binary with IMGM’s new Biorad QX200™ Droplet Digital™ PCR service allows you quantifying target sequences at single molecule level while increasing exactness of your traditional PCR applications and opening new areas of usage.
In the digital PCR workflow, the reaction is partitioned before amplification in approximately 20’000 separate droplets. Due to appropriate dilution, each droplet contains exactly one (positive droplets) or none (negative droplets) target sequence copy. The thus separated target copies are amplified up to 1 million-fold by TaqMan PCR generating fluorescence-based probe signals in the positive droplets only. Now things are getting digital as the initial number of single target molecules is translated into clear, binary information units: fluorescence-positive and -negative droplets. Their fluorescence signals are used to determine the ratio of positive and negative droplets which allows calculating back to the absolute DNA amount in the initial sample. As in this approach counting fluorescence signals means counting single molecules, no reference sample or standard curve is required for absolute quantification of the target DNA. The confinement of amplification and signal detection to single molecule level in digital PCR massively increases accuracy and sensitivity compared to conventional PCR based on collective endpoint or real time measurements.
Resulting digital PCR applications, unthinkable in this accuracy with conventional PCR, are e.g. detection of single nucleotide mutations, rare alleles or copy number variation as well as absolute quantification of DNA or gene expression. Convenient analysis is made possible by a huge number of predesigned PrimePCR™ ddPCR™ Assays available from Biorad for many common targets. Even difficult samples like cell free DNA or FFPE samples containing low amounts of DNA or complex mixtures are made accessible as the droplet approach efficiently focuses the PCR reaction and reduces inhibitory effects.
Get digital – YES or NO?Go for YES and profit from IMGM’s new Digital PCR Service to analyze your samples waiting for an accurate and sensitive method of DNA quantification or variant detection.Get in touch with our team via our contact sheet or call +49.89.4524667.0. For more information on IMGM’s service portfolio check out our homepage www.IMGM.com
The IMGM Team
Central, Biomarker and Speciality Labs Summit
Park Plaza Victoria London, UK
February 24-25, 2015
German Society for Gene Therapy (DG-GT e.V.), XXI. Annual Meeting
University Vienna, Austria
February 26-28, 2015
qPCR & NGS 2015 Event
Advanced Molecular Diagnostics for Biomarker Discovery