RNA Sequencing and FFPE samples: Two natural enemies finally reconciled by IMGM´s TruSeq RNA Access Service
Carved in stone, painted on canvas or scratched in vinyl - expressions of human thoughts and feelings have been conserved over generations and been ever since an easily accessible treasure of knowledge and inspiration. When it comes to expression of genes conserved in FFPE, things are different. Given the degradation of RNA to shreds and pieces, the scientific treasure of potential insights and discoveries resting worldwide in FFPE archives is everything but easy to unearth. Read on and find out how IMGM’s new FFPE RNA Sequencing Service using Illumina TruSeq RNA Access technology helps you unraveling new findings by enabling robust and reliable analysis of gene expression in your FFPE or other low-quality RNA samples.
High sample amount input requirements and highly variable results are buzzwords you don’t want to hear when planning the next sequencing experiment with your precious and irreplaceable FFPE or partly degraded RNA sample collection. Unfortunately, classic approaches will take you straight to those two obstacles which hamper immensely the effective gene expression analysis of challenging RNA samples.
The new Illumina TruSeq RNA Access kit, now part of IMGM’s service portfolio, offers straightforward and high quality solutions to overcome these problems. Even low-quality RNA can be used as input material and as little as 20-100 ng of RNA are required. Regardless of low quality and low RIN values, RNA can be evaluated for its usability by the newly established Illumina DV200 quality parameter. Subsequent stranded library preparation is key and enhances quality by a clever enrichment step comparable to exome capture: Long, high quality fragments of coding regions are amplified by >425’000 probes targeting 21’415 genes of NCBI37/hg19 representing 98.3% of RefSeq exome.
By depletion of degraded RNA, the Illumina TruSeq RNA Access approach diminishes variability while greatly reducing required sequencing depth. First published Illumina results show that 25 mio reads per sample of a TruSeq Access library provide transcript coverage comparable to 200 mio reads of a conventional RNA library from FFPE samples.
Start accessing your FFPE samples efficiently and unravel information about expression and fusion of genes with IMGM´s FFPE RNA Sequencing Service covering all steps from sample submission to expert bioinformatics analysis.
How many scientific treasures are waiting in your FFPE archives to be revealed by RNA sequencing? Get in touch with our team via our contact sheet or call +49.89.4524667.0. For more information on IMGM’s service portfolio check out our homepage www.IMGM.com
The IMGM Team
qPCR & NGS 2015 Event
Advanced Molecular Diagnostics for Biomarker Discovery