qPCR - Quantitative Polymerase Chain Reaction

qPCR - Quantitative Polymerase Chain Reaction

At IMGM, we offer qPCR analysis on the Life Technologies ViiA7 Real-Time PCR System using TaqMan or SYBR Green chemistry.  We work with 96- as well as 384-well blocks and use either general or pre-spotted microfluidic plates in different formats. Thereby, we are able to provide you with ideal solutions from small- to large-scale projects.

The IMGM team can help you with designing your qPCR project perfectly. We generate first-class data and offer bioinformatics data analysis to provide you with high-value information about your samples.

IMGM Laboratories GmbH holds a certificate of Good Laboratory Practice (GLP) compliance for quantitative nucleic acid sequence detection issued by the Bavarian Health and Food Safety Authority.

IMGM Laboratories GmbH also holds an accreditation for gene expression analysis using real-time PCR according to DIN EN ISO / IEC 17025 awarded by the DAkkS (Deutsche Akkreditierungsstelle GmbH).

The portfolio of qPCR services at IMGM include:

  • Assay design and validation
  • Gene/miRNA expression analysis
  • Absolute quantification e.g. for biodistribution studies
  • SNP genotyping
  • CNV analysis

In addition, we offer custom solutions where we use our expertise to counsel you about the optimal approach for your research focus.

ddPCR – Bio-Rad QX200 Droplet Digital PCR

In IMGM’s digital PCR workflow, the reaction is partitioned in approximately 20’000 separate droplets. Thereby, each droplet contains exactly one (positive droplets) or no (negative droplets) target sequence copy. These molecules are then amplified up to 1 million-fold by TaqMan PCR generating fluorescence signals in the positive droplets only.

These signals are used to determine the ratio of positive and negative droplets, which allows calculating back to the absolute DNA amount in the initial sample. No reference sample or standard curve is required for absolute quantification of the target DNA.

Resulting digital PCR applications with increased accuracy compared to conventional PCR are e.g. detection of single nucleotide mutations, rare alleles or copy number variation as well as absolute quantification of DNA or gene expression. Convenient analysis is made possible by a huge number of predesigned PrimePCR ddPCR Assays available from Bio-Rad for common targets. Even difficult samples like cell free DNA or FFPE material are accessible as the droplet approach reduces inhibitory effects.

If you have a question about our qPCR or ddPCR services or would like to request a quote for a specific project, our expert team will be pleased to get in touch with you. Contact us.

 

Go for enhanced analytical power!
Entrust IMGM with your next qPCR project.